不同方法提取马氏珠母贝外套膜胶原蛋白理化性质比较     

林海生  王雯  秦小明  曹文红  章超桦  高加龙  郑惠娜  陈忆宾  伍彬 《广东海洋大学学报》2022,(1)

【目的】系统分析不同方法提取的马氏珠母贝(Pinctada martensii)外套膜胶原蛋白的理化性质及生物学功能。【方法】分别采用酸-酶法和热水法从外套膜中提取得到两种胶原蛋白(A-PSC(Pm)和HSC(Pm)),对其氨基酸组成、微观结构及热变性温度等理化特性进行比较分析。【结果】HSC(Pm)提取率高于A-PSC(Pm);HSC(Pm)中,Gly、Glu与Arg相对含量较高,分别为20.16%、17.57%和10.07%;而A-PSC(Pm)中,Glu、Asp与Arg相对含量为19.67%、11.63%和10.07%;紫外光谱和红外光谱分别显示,两种胶原蛋白均在220 nm左右有强吸收峰,且具有典型的胶原蛋白特征带(酰胺A、B、Ⅰ、Ⅱ和Ⅲ带);扫描电镜结果显示,A-PSC(Pm)呈致密多孔网状结构,HSC(Pm)则基本为薄片状;DSC分析结果表明,HSC(Pm)具有更好的热稳定性。【结论】酸-酶法及热水法提取的胶原蛋白均具有类V型胶原蛋白特性,其结构特征及其理化性质均存在差异,可根据需求选择特定的胶原蛋白提取方法用以开发不同类型的产品。  相似文献   

5000 m新型能源勘探智能钻探装备与技术研究          下载免费PDF全文

朱恒银  王强  刘兵  陈云召  冯建宇  乌效鸣 《探矿工程》2022,49(1):110-119

结合我国地下新型能源勘探工作的重大需求,开展了“5000 m新型能源勘探智能钻探装备与技术”的研究,取得了5000 m多功能交流变频电动钻机、多参数孔底自动监测装置、高温环保泥浆及泥浆性能自动测量装置、取心和无心钻进不提钻互换钻具等系列成果,填补了国内空白,推动了新型能源深部钻探技术向智能化方向发展。  相似文献   

新生代辉绿岩侵入对围岩物性的影响:以苏北盆地洋心次凹为例     

王凯  刘汇川  任威威  李文奇  于志琪 《现代地质》2022,36(6):1563-1573

传统观念认为岩浆活动区是油气勘探的禁区,但现今理论研究与实践勘探发现岩浆侵入作用能够显著改造围岩物性。目前岩浆侵入对围岩具体的影响认识不清,查明岩浆侵入影响围岩物性的机理对油气勘探具有重要意义。以苏北盆地洋心次凹的丰页1井为例,结合测井、地震及岩心等资料重点分析辉绿岩侵入对围岩储层物性与储集空间的改造,深入讨论其影响机制。苏北地区丰页1井的辉绿岩在吴堡运动期(约59 Ma)开始顺层侵入,侵入体中心相为辉长岩,侵入体边缘相为辉绿岩,变质相为板岩,上变质带厚度大于下变质带厚度。围岩由非渗透性泥岩转变为油气储层,储集空间为辉绿岩侵入和泥岩变质过程中产生的裂缝与孔隙。辉绿岩的侵入显著提高围岩储层物性,主要的机制有变质固结、热液破裂、冷凝收缩和溶蚀作用4种,其中热液破裂和溶蚀作用对储层的形成最为关键。  相似文献   

火山灰增强微生物固化砂土效果的试验研究     

熊雨  邓华锋  李建林  程雷  朱文羲 《岩土力学》2022,43(12):3403-3415

为了提升微生物固化砂土的效果,考虑火山灰的多孔结构及活性特征,设计进行了火山灰增强微生物诱导碳酸钙沉淀（MICP）固化砂土试验,综合宏观物理力学试验和微细观检测,系统分析了火山灰对微生物固化砂土的增强效果及增强机制。结果表明：（1）火山灰能够显著提高砂土微生物加固过程中的固菌率和胶结物产量,火山灰掺量在10%左右达到最佳,与常规MICP相比,固菌率提高了118.28%,胶结物生成量提高了29.55%。（2）火山灰的掺入提高了固化体的抗压强度和抵抗变形的能力,不同围压下固化体的抗压强度提升了52.26%～62.96%,破坏时的应变增加了100.00%～112.58%。（3）火山灰掺入后,固化体的孔隙大小及孔隙率明显减小,整体的密实性及抗渗性能进一步提升,孔隙率从20.12%减小为14.17%,渗透系数降低了一个数量级。（4）火山灰对微生物固化砂土的增强机制主要包括3个方面,一方面,火山灰在砂颗粒间起到了良好的充填作用,大幅减少了颗粒间的大孔隙,使得固化体的密实性增强;另一方面,火山灰良好的吸附作用有效提高了试样内细菌的含量,使固化体碳酸钙的产量及分布的均匀性均增加;第3方面,火山灰中的活性物质参与反应生成的胶凝物质与碳酸钙晶体形成复合凝胶体,使得固化体的胶结性能和密实程度进一步增强。  相似文献   

不同初始饱和度红砂岩冻融后物理力学性质研究          下载免费PDF全文

沈世伟  吴飞  甘霖  姜满 《探矿工程》2021,48(1):120-128

对不同初始饱和度红砂岩冻融循环前后进行物理及力学试验研究,探讨初始饱和度对红砂岩冻融损伤的影响。本文设定红砂岩试样初始饱和度为20%、40%、60%、80%、100%,冻融次数设定为20次,对冻融前后试样分别测定质量、纵波波速以及进行单轴压缩试验。试验结果表明：1）冻融后不同初始饱和度红砂岩的物理性质发生变化,纵波波速降低、质量减小,但变化程度不同;2）随饱和度的增大,试样经冻融后峰值强度和弹性模量均呈降低趋势,但只有饱和度大于60%时,降低趋势较明显。本文研究为寒区隧道及地下工程建设以及岩土地质灾害监测与治理提供理论依据和试验基础。  相似文献   

冻土机械切削破碎机理的研究进展   总被引：1，自引：1，他引：0  

李龙  周琴  张凯  凌雪  张在兴  李耀 《冰川冻土》2021,43(2):638-649

冻土开挖困难、破碎效率低是高寒地区工程建设、地基施工等面临的技术难题。冻土机械切削破碎是冻土开挖的主要方法,其机理研究是提高冻土破碎效率的前提和基础。首先总结了温度、含水率、围压等对冻土复杂力学特性的影响,进而调研分析了冻土机械切削破碎的典型切削力学模型,发现冻土切削机械破碎模式不仅与冻土力学特性密切相关,也与切削参数和刀具结构直接相关,冻土切削过程中存在着最优的切削前角（30°~60°）,且深切削和浅切削时冻土内部受力方式存在差异也会导致破坏形式的不同;温度、含水率、围压所造成的冻土力学性能变化会直接导致冻土破坏过程和切削破碎机理的改变,冻土强度随着温度降低表现出先升高然后保持稳定的特性,随着含水率升高呈现出先升高后降低的趋势,冻土破碎存在脆性、塑脆过渡及塑性等不同破坏形式。通过系统总结冻土切削破碎机理研究进展,进一步明确了冻土力学性质主要影响因素、变化特点及其切削破坏损伤特征,为冻土机械切削破碎的切削参数和切削具结构优化提供了设计依据。  相似文献   

高寒冻土区生物结皮对土壤理化属性的影响   总被引：1，自引：0，他引：1  

明姣  盛煜  金会军  张泽  杜玉霞 《冰川冻土》2021,43(2):601-609

生物结皮是高寒地区地被层的重要组分之一。其作为地表特殊的结构层,能够改变地表结构及土壤理化属性,从而影响冻土环境。迄今为止,关于青藏高原高寒生态系统中生物结皮对土壤理化属性的影响尚不清楚。以青藏高原高寒冻土区生物结皮为研究对象,初步研究了生物结皮的特征及其对土壤理化属性的影响。结果表明：生物结皮在高寒草甸退化过程中广泛发育,主要以藻结皮为主,其盖度可达37.3%~51.7%,结皮层平均厚度为12.6 mm。由于生物结皮的发育,高寒地区5~20 cm土层粉粒含量有所增加,但差异不显著,而结皮层土壤田间持水量相比于裸地表层（2 cm）增加了10%~40%,结皮层容重较裸地降低了30%;两种类型藻结皮均显著增加了结皮层及其下0~20 cm土层土壤有机质,而深色藻结皮增加了结皮层及其下0~20 cm土层土壤全氮含量,浅色藻结皮仅增加了结皮层土壤全氮含量,对其下0~20 cm土层土壤全氮含量没有显著影响;生物结皮对土壤pH没有显著影响;生物结皮是高寒生态系统植被退化过程中的关键环节。研究结果为揭示生物结皮在高寒生态系统中发挥重要生态功能提供依据。  相似文献   

Quantitative characterization of different coal rank reservoirs permeability based on NMR and X-CT technology     

GUO Guangshang  XING Liren  LI Hao 《中国地质调查》2021,7(6):103-108

With the increasing development of coalbed methane (CBM) field, the quantitative characterization and evaluation of coal reservoir physical properties is becoming more and more important to CBM scale development. In order to solve the limitations of conventional methods for testing coal reservoir physical properties, the authors used the high-tech technologies such as nuclear magnetic resonance technology and CT scanning technology to effectively solve the problems of in-situ and integrity of rock samples and acquire the porosity and permeability. Nuclear magnetic resonance and CT scanning experiments of coal samples were carried out to rapidly obtain the pore type, pore size distribution and connectivity, effective porosity, spatial distribution of pore fissures and other refined coal reservoir physical parameters, based on the samples of middle and low rank coal in Shenfu block and high rank coal in Shizhuangnan block. So a set of quantitative characterization analysis technology that can be applied to coal reservoirs with different coal rank was formed.  相似文献   

Geology and mineralization of the Sanshandao supergiant gold deposit (1200 t) in the Jiaodong Peninsula,China: A review     

《China Geology》2021,4(4):686-719

The Jiaodong Peninsula in Shandong Province, China is the world’s third-largest gold metallogenic area, with cumulative proven gold resources exceeding 5000 t. Over the past few years, breakthroughs have been made in deep prospecting at a depth of 500–2000 m, particularly in the Sanshandao area where a huge deep gold orebody was identified. Based on previous studies and the latest prospecting progress achieved by the project team of this study, the following results are summarized. (1) 3D geological modeling results based on deep drilling core data reveal that the Sanshandao gold orefield, which was previously considered to consist of several independent deposits, is a supergiant deposit with gold resources of more than 1200 t (including 470 t under the sea area). The length of the major orebody is nearly 8 km, with a greatest depth of 2312 m below sea level and a maximum length of more than 3 km along their dip direction. (2) Thick gold orebodies in the Sanshandao gold deposit mainly occur in the specific sections of the ore-controlling fault where the fault plane changes from steeply to gently inclined, forming a stepped metallogenic model from shallow to deep level. The reason for this strong structural control on mineralization forms is that when ore-forming fluids migrated along faults, the pressure of fluids greatly fluctuated in fault sections where the fault dip angle changed. Since the solubility of gold in the ore-forming fluid is sensitive to fluid pressure, these sections along the fault plane serve as the target areas for deep prospecting. (3) Thermal uplifting-extensional structures provide thermodynamic conditions, migration pathways, and deposition spaces for gold mineralization. Meanwhile, the changes in mantle properties induced the transformation of the geochemical properties of the lower crust and magmatic rocks. This further led to the reactivation of ore-forming elements, which provided rich materials for gold mineralization. (4) It can be concluded from previous research results that the gold mineralization in the Jiaodong gold deposits occurred at about 120 Ma, which was superimposed by nonferrous metals mineralization at 118–111 Ma. The fluids were dominated by primary mantle water or magmatic water. Metamorphic water occurred in the early stage of the gold mineralization, while the fluid composition was dominated by meteoric water in the late stage. The S, Pb, and Sr isotopic compositions of the ores are similar to those of ore-hosting rocks, indicating that the ore-forming materials mainly derive from crustal materials, with the minor addition of mantle-derived materials. The gold deposits in the Jiaodong Peninsula were formed in an extensional tectonic environment during the transformation of the physical and chemical properties of the lithospheric mantle, which is different from typical orogenic gold deposits. Thus, it is proposed that they are named “Jiaodong-type” gold deposits.©2021 China Geology Editorial Office.  相似文献   

2010—2016年江西省暖季短时强降水特征分析   总被引：2，自引：0，他引：2  

付超  谌芸  朱克云  单九生  曾智琳 《气象》2019,45(9):1238-1247

利用江西省2010—2016年5—9月1597个观测站逐小时降水资料对江西省短时强降水进行统计分析。采用REOF将降水场划分为5个区域：赣北南部（Ⅰ区）,抚州市及赣州中部（Ⅱ区）,赣北北部（Ⅲ区）,赣南南部、北部（Ⅳ区）以及赣中西部（Ⅴ区）。短时强降水高频区主要分布在山地及河谷附近,分别为湘赣交界罗霄山脉东侧、武夷山西侧、信江河谷、乐安河谷和昌江河谷。河谷附近短时强降水频次以昌江河谷最高（16.9次/a）,山地附近最高在罗霄山脉东侧（12.6次/a）,极端短时强降水分别位于上饶市东北部山区（3.7次/a）及九岭山南侧的锦江河谷（3.3次/a）。短时强降水主要发生在5月第3候,6、7月第3~4候以及8月第2~3候。Ⅳ、Ⅴ区具有单峰型的日变化特征;Ⅰ、Ⅱ、Ⅲ区具有双峰型的日变化特征。主峰基本集中在下午17时;次峰在上午08—10时。短时强降水对暴雨贡献率基本在40%以上,Ⅰ、Ⅱ区的暴雨天气过程将近一半是由短时强降水贡献的。信江河谷是暴雨雨量中心,但并不是短时强降水雨量中心;昌江河谷与武夷山西麓既是暴雨中心也是短时强降水中心。  相似文献